The inspiratory activity of respiratory center and respiratory muscles strength after COVID-19.

The respiratory pump that provides pulmonary ventilation includes the respiratory center, peripheral nervous system, chest and respiratory muscles. The aim of this study was to evaluate the activity of the respiratory center and the respiratory muscles strength after COVID-19 (COronaVIrus Disease 2019). Methods. The observational retrospective cross-sectional study included 74 post-COVID-19 patients (56 (76%) men, median age - 48 years). Spirometry, body plethysmography, measurement of lung diffusing capacity (DLCO), maximal inspiratory and expiratory pressures (MIP and MEP), and airway occlusion pressure after 0.1 sec (P0.1) were performed. In addition, dyspnea was assessed in 31 patients using the mMRC scale and muscle strength was assessed in 27 of those patients using MRC Weakness scale. Results. The median time from the COVID-19 onset to pulmonary function tests (PFTs) was 120 days. The total sample was divided into 2 subgroups: 1 - P0.1 <= 0.15 kPa (norm), 2 - > 0.15 kPa. The lung volumes, airway resistance, MIP, and MEP were within normal values in most patients, whereas DLCO was reduced in 59% of cases in both the total sample and the subgroups. Mild dyspnea and a slight decrease in muscle strength were also detected. Statistically significant differences between the subgroups were found in the lung volumes (lower) and airway resistance (higher) in subgroup 2. Correlation analysis revealed moderate negative correlations between P0.1 and ventilation parameters. Conclusion. Measurement of P0.1 is a simple and non-invasive method for assessing pulmonary function. In our study, an increase in P0.1 was detected in 45% of post-COVID-19 cases, possibly due to impaired pulmonary mechanics despite the preserved pulmonary ventilation as well as normal MIP and MEP values.Copyright © Savushkina O.I. et al., 2023.

Tomisaku Kawasaki: identified and named Kawasaki disease

"Kawasaki was an icon in the paediatric world,” Jane Burns, professor and director of the Kawasaki Disease Research Centre at the University of California San Diego School of Medicine, told The BMJ. In 1949 he became a staff paediatrician at the Red Cross Hospital outside Tokyo and began to undertake research. ” "Ten years after starting at the Japanese Red Cross Central Hospital (now the Japanese Red Cross Medical Centre) in Tokyo, I examined on 5 January 1961 a boy aged four years and three months with a curious clinical symptom complex I had never seen,” he explained.3 "The patient had a high fever of about two weeks' duration, marked bilateral conjunctival hyperaemia without discharge, reddening dry fissured lips, diffuse redness of the mucous membrane of oral cavity, strawberry like tongue, left non-purulent cervical adenopathy, polymorphous erythema on the body, and marked redness of palms and soles, with indurative oedema of hands and feet following desquamation from the fingertips.”

The role of the oral cavity in SARS-CoV-2- and other viral infections.

OBJECTIVE: This study aims to review the role of the oral cavity in SARS-CoV-2- and other viral upper respiratory tract infections. MATERIAL AND METHODS: Data reviewed in the text have been researched online and also reflect personal expertise. RESULTS: Numerous respiratory and other viruses replicate in the oral cavity and are transmitted via aerosols (< 5 µm) and droplets (> 5 µm). SARS-CoV-2 replication has been documented in the upper airways as well as in oral mucosa and salivary glands. These sites are also virus reservoirs that can infect other organs, e.g., the lungs and gastrointestinal tract, as well as other individuals. Laboratory diagnosis of viruses in the oral cavity and upper airways focuses on real-time PCR; antigen tests are less sensitive. For screening and monitoring infections, nasopharyngeal and oral swabs are tested; saliva is a good and more comfortable alternative. Physical means like social distancing or masks have been proven successful to reduce the risk of infection. Both wet-lab and clinical studies confirm that mouth rinses are effective against SARS-CoV-2 and other viruses. Antiviral mouth rinses can inactivate all viruses that replicate in the oral cavity. CONCLUSIONS: The oral cavity plays an important role in viral infections of the upper respiratory tract: it serves as a portal of entry, a site of replication, and a source of infection by droplets and aerosols. Physical means but also antiviral mouth rinses can help reduce the spread of viruses and contribute to infection control.

The inspiratory activity of respiratory center and respiratory muscles strength after COVID-19

The respiratory pump that provides pulmonary ventilation includes the respiratory center, peripheral nervous system, chest and respiratory muscles. The aim of this study was to evaluate the activity of the respiratory center and the respiratory muscles strength after COVID-19 (COronaVIrus Disease 2019). Methods. The observational retrospective cross-sectional study included 74 post-COVID-19 patients (56 (76%) men, median age – 48 years). Spirometry, body plethysmography, measurement of lung diffusing capacity (DLCO), maximal inspiratory and expiratory pressures (MIP and MEP), and airway occlusion pressure after 0.1 sec (P0.1) were performed. In addition, dyspnea was assessed in 31 patients using the mMRC scale and muscle strength was assessed in 27 of those patients using MRC Weakness scale. Results. The median time from the COVID-19 onset to pulmonary function tests (PFTs) was 120 days. The total sample was divided into 2 subgroups: 1 – P0.1 ≤ 0.15 kPa (norm), 2 – > 0.15 kPa. The lung volumes, airway resistance, MIP, and MEP were within normal values in most patients, whereas DLCO was reduced in 59% of cases in both the total sample and the subgroups. Mild dyspnea and a slight decrease in muscle strength were also detected. Statistically significant differences between the subgroups were found in the lung volumes (lower) and airway resistance (higher) in subgroup 2. Correlation analysis revealed moderate negative correlations between P0.1 and ventilation parameters. Conclusion. Measurement of P0.1 is a simple and non-invasive method for assessing pulmonary function. In our study, an increase in P0.1 was detected in 45% of post-COVID-19 cases, possibly due to impaired pulmonary mechanics despite the preserved pulmonary ventilation as well as normal MIP and MEP values. © Savushkina O.I. et al., 2023.

The inspiratory activity of respiratory center and respiratory muscles strength after COVID-19.

The respiratory pump that provides pulmonary ventilation includes the respiratory center, peripheral nervous system, chest and respiratory muscles. The aim of this study was to evaluate the activity of the respiratory center and the respiratory muscles strength after COVID-19 (COronaVIrus Disease 2019). Methods. The observational retrospective cross-sectional study included 74 post-COVID-19 patients (56 (76%) men, median age - 48 years). Spirometry, body plethysmography, measurement of lung diffusing capacity (DLCO), maximal inspiratory and expiratory pressures (MIP and MEP), and airway occlusion pressure after 0.1 sec (P0.1) were performed. In addition, dyspnea was assessed in 31 patients using the mMRC scale and muscle strength was assessed in 27 of those patients using MRC Weakness scale. Results. The median time from the COVID-19 onset to pulmonary function tests (PFTs) was 120 days. The total sample was divided into 2 subgroups: 1 - P0.1 <= 0.15 kPa (norm), 2 - > 0.15 kPa. The lung volumes, airway resistance, MIP, and MEP were within normal values in most patients, whereas DLCO was reduced in 59% of cases in both the total sample and the subgroups. Mild dyspnea and a slight decrease in muscle strength were also detected. Statistically significant differences between the subgroups were found in the lung volumes (lower) and airway resistance (higher) in subgroup 2. Correlation analysis revealed moderate negative correlations between P0.1 and ventilation parameters. Conclusion. Measurement of P0.1 is a simple and non-invasive method for assessing pulmonary function. In our study, an increase in P0.1 was detected in 45% of post-COVID-19 cases, possibly due to impaired pulmonary mechanics despite the preserved pulmonary ventilation as well as normal MIP and MEP values.Copyright © Savushkina O.I. et al., 2023.

Colliding Pandemics and CoViD-19

Cases of the respiratory syncytial virus (RSV), monkeypox virus (MPXV), and avian influenza A Virus (IAV) have increased during our current prolonged Corona Virus Disease 2019 (CoViD-19) pandemic. The rise of these viral infectious diseases may be associated or even inter-dependent with acute, latent or recurrent infection with Systemic Acute Respiratory Syndrome Corona virus-2 (SARS-CoV2). The nonsensical neologism 'tripledemic' was tentatively introduced to describe the confluent nature of these trends (epidemic comes from two Greek words: epi=on, about, demos=people;pandemic is also derived from Ancient Greek: pan=all, demos=people;but 'tripledemic' would derive from Latin triplus=three, Greek demos=people, and would at best signify 'three countries, three peoples', but certainly not the current threat of confluence of three, or perhaps more pandemics). Emerging evidence suggests that monkey pox and CoViD-19, among several other viral diseases, produce significant observable manifestations in the oral cavity. From a clinical standpoint, dentists and dental personnel may be among the first health professionals to encounter and diagnose clinical signs of converging infections. From the immune surveillance viewpoint, viral recombination and viral interference among these infectious diseases must be examined to determine the potential threat of these colliding pandemics.

Triple Trouble: Disseminated Penicilliosis in a Cancer patient with COVID-19

Penicilliosis is a fungal infection caused by the fungus Penicillium marneffei or Talaromyces marneffei . Penicillosis is commonly seen in immunocompromised patients such as in HIV(AIDS). Herein, we present a case of penicilliosis in an oral cavity cancer patient who was admitted for the management of SARS-CoV-2 infection at our hospital. A 50-year-old male patient operated on for squamous cell carcinoma of the oral cavity who completed his adjuvant chemoradiation 2 months ago, presented to our hospital with dry cough for more than 3 weeks. His nasopharyngeal swab was positive for the severe acute respiratory distress syndrome (SARS-CoV-2). During his hospital stay for SARS-CoV-2 infection, he was diagnosed with disseminated penicilliosis. The patient was treated with intravenous antifungals caspofungin and voriconazole. However, he succumbed to disseminated fungal sepsis. This case highlights the need to consider penicilliosis as a possible opportunistic pathogen, especially in immunocompromised patients such as cancer.

Oral lesions in patients with severe COVID-19 in the National Institute of Respiratory Diseases in Mexico: Case series

Introduction SARS-CoV-2 is a neurotropic, mucotropic, and sialotropic virus that can affect the salivary glands' function, taste sensations, smell, and oral mucosa integrity.1 The oral cavity is a perfect habitat for SARS-CoV-2 invasion due to the special affinity the virus has for cells with angiotensinconverting enzyme (ACE2) receptors, such as those from the respiratory tract, oral mucosa, tongue, and salivary glands. Aphthous lesions with necrosis and hemorrhagic crusts have been described to manifest more regularly in older adults with immunosuppression and severe COVID-19 infection;one hypothesis for the development of aphthous lesions and/or ulcers is given due to the ACE2 receptor and the SARS-CoV-2 interaction, which could alter the epithelial lining of salivary glands and keratinocytes, causing lesions in the oral cavity.4 At the same time, different etiological factors such as infections, immune system alterations, and direct trauma to the oral mucosa or epithelium,5 may be related to the stress of a prolonged hospital stay.6 Including pressure in the oral cavity conditioned by the prone position, malposition of the endotracheal tube (mainly in the corners of the lips),7 medication-related nutritional deficiencies8 such as lopinavir, and ritonavir, oseltamivir, hydroxychloroquine, among others.9-12 Thrombotic vasculopathy secondary to COVID-19 has also been described, induced by system mediators in the microvascular walls, which impairs endothelial cells, and activates coagulation factors13 and a possible hypersensitivity reaction of the mucosa to the presence of SARS-CoV-2 in the epithelium14,15;there is also the hypothesis that it could be associated with an exanthem pattern induced by the inflammatory action of the SARSCoV-2 virus,16 presented as increased levels of cytokines (including interleukin-1, tumor necrosis facto-a), and arachidonic acid metabolites (prostaglandins) secondary to the stem cell factor production and the basic fibroblast growth factor of keratinocytes from the basal layer, in relation to post-inflammatory pigmentations that could appear in areas previously affected by trauma or chronic inflammation.17 Oral manifestations in COVID-19 patients appear, on many occasions, even before respiratory symptoms, although exanthematic lesions observed in COVID-19 patients can also be observed in other viral processes. Physical examination revealed a patient in a supine position with orotracheal intubation and orogastric tube, with aphthous-type ulcers, some of them had blood crusts of different sizes on the lower lip (both skin and mucosa), dorsum, and lateral edge of the tongue, gum, and vestibular fornix (Figure 3). Initial physical examination shows the patient in a supine position supported by high-flow nasal prongs, upper and lower lips edema and ulcer-like lesions with hematic crusts on both lips (Figure 4), topical management with steroids and GELCLAIRE® Oral Gel (glycyrrhetinic acid and polyvinylpyrrolidone) is observed.

Oral and Dental Infections: Virus

The oral cavity is a prime entering point for all types of microbiota, including viruses. The majority of viruses that enter cause no symptoms. Some are potentially pathogenic, but focus on other organs. Only a few use the cells associated with the oral cavity for replication, but their activity may affect oral health. Papillomaviruses are probably involved in oral cancer, and several viruses can take part in ulceration. Viruses of the herpes family may have an additional role in the development of periodontitis. This chapter offers a brief introduction to virology before discussing the role of the more relevant viruses in oral diseases and their treatment. Local anti-herpes medications are used for sores on the lips, while treatment of similar herpes blister or ulcers inside the mouth would require systemic administration. This is rarely done, but related medicine has been used successfully in connection with periodontitis. Vaccination against papillomaviruses appears to decrease the prevalence of oral cancer. © 2022 Elsevier Inc. All rights reserved.

Correlation of Redondovirus and Entamoeba gingivalis Detections in the Human Oral Cavity Suggests That This Amoeba Is Possibly the Redondovirus Host.

The virome of the human oral cavity and the relationships between viruses and diseases such as periodontitis are scarcely deciphered. Redondoviruses were reported in the human oral cavity in 2019, including in periodontitis patients. Here, we aimed at detecting redondoviruses and at searching for a potential viral host in human saliva. Non-stimulated saliva was collected between December 2020 and June 2021. These samples were tested using real-time PCR regarding the presence of redondovirus and Entamoeba gingivalis DNA. Similarity searches were performed using BLAST against eukaryotic and prokaryotic sequences from GenBank. The redondovirus DNA was detected in 46% of the 28 human saliva samples. In addition, short fragments of redondovirus genomes were detected in silico within Entamoeba sequences. Finally, Entamoeba gingivalis DNA was detected in 46% of the 28 saliva samples, with a strong correlation between redondovirus DNA and E. gingivalis DNA detections, in 93% of the cases. Regarded together, these findings and previous ones strongly support the presence of redondoviruses in the human oral cavity and their association to E. gingivalis as their likely host.

Oral candidiasis combined with COVID-19 (literature review)

The oral cavity is known as a potential reservoir for respiratory pathogens. Candida fungi are normally present in 40-60% of people and are conditionally pathogenic. It is known that a single isolation of yeast-like fungi from the mucous membrane in an amount of more than 1×103 CFU/ml is considered as candidiasis. SARS-CoV-2 penetrates the body due to angiotensin-converting enzyme 2 (ACE2), a type I membrane protein that is expressed on epithelial cells of the oral cavity, in salivary glands, tongue, buccal and gingival epithelial cells. The course of COVID‐19 is determined by age, the state of oral hygiene, the presence of concomitant diseases and bad habits - hypertension, diabetes mellitus, cardiovascular pathology, taking various medications, smoking. The most frequent manifestation of the imbalance of microbial flora in the oral cavity is candidiasis, which can be covid-associated both during the active phase of COVID-19 and in the post-covid period. Some clinical factors, such as prolonged stay in the intensive care unit, the use of central venous catheters and the use of broad-spectrum antibiotics, may dominate immune-mediated mechanisms that increase susceptibility to candidemia in COVID-19 conditions. Publications devoted to covid-associated candidiasis reflect typical and atypical clinical complaints of patients: burning, soreness, the presence of whitish plaques on the tongue, as well as reddish plaques on the lower lip. The aim of the work is to systematize the literature data on the relationship of oral candidiasis and SARS-CoV-2. Materials and methods. The search and analysis of scientific publications in the electronic databases PubMed, eLibrary from 2019 to 2022 was carried out. Results. As a result of the literature analysis, most authors describe clinical cases of oral candidiasis combined with COVID-19. The published data demonstrate the atypical nature of situations and manifestations of oral candidiasis, as well as the problems of a particular patient. In this regard, the question of the need for therapeutic and preventive measures in patients during these periods in order to prevent the development of candidiasis (both local and systemic) remains open at the moment. Conclusion. Mycotic lesions of the oral cavity are relatively rarely described in the scientific literature and are of a statistical nature. Clinical cases provide an opportunity for a more detailed study of this issue. At the moment, the question of carrying out therapeutic and preventive measures in patients in the acute phase of COVID-19 and in the post-covid period in order to prevent the development of candidiasis remains open. © 2020 Clinical Dentistry LLC. All Rights Reserved.

Assessment of SARS-CoV-2 entry in gingival epithelial cells expressing CD147.

SARS-CoV-2, the causative agent of the debilitating COVID-19, is mainly transmitted by first infecting nose and lung epithelial cells. The mouth is also believed to be a viral portal site since certain types of oral epithelial cells were shown to express ACE2 receptor. However, it is unclear whether oral epithelial cells are directly infected by SARS-CoV-2. In this study, we addressed whether epithelial cells of the oral gingiva were susceptible to infection. Interestingly, we found that KRT5+ and KRT18+ gingival epithelial cells do not express ACE2 but highly express TMPRSS2 and Furin as well as CD147, which was proposed to be an alternative receptor for SARS-CoV-2. However, using SARS-CoV-2 pseudoviruses containing the spike protein, we observed that gingival epithelial cells were not susceptible to infection due to the lack of ACE2 expression and the inability of CD147 to mediate viral entry. These results strongly suggest that epithelial cells from the gingiva are not susceptible to SARS-CoV-2 and CD147 is not a receptor for the SARS-CoV-2 virus. The susceptibility of oral cells from other oral structures under healthy and pathological conditions still needs to be confirmed to better understand the role of the oral cavity in COVID-19 infection and transmission.

The Role of the Plasminogen/Plasmin System in Inflammation of the Oral Cavity.

The oral cavity is a unique environment that consists of teeth surrounded by periodontal tissues, oral mucosae with minor salivary glands, and terminal parts of major salivary glands that open into the oral cavity. The cavity is constantly exposed to viral and microbial pathogens. Recent studies indicate that components of the plasminogen (Plg)/plasmin (Pm) system are expressed in tissues of the oral cavity, such as the salivary gland, and contribute to microbial infection and inflammation, such as periodontitis. The Plg/Pm system fulfills two major functions: (a) the destruction of fibrin deposits in the bloodstream or damaged tissues, a process called fibrinolysis, and (b) non-fibrinolytic actions that include the proteolytic modulation of proteins. One can observe both functions during inflammation. The virus that causes the coronavirus disease 2019 (COVID-19) exploits the fibrinolytic and non-fibrinolytic functions of the Plg/Pm system in the oral cavity. During COVID-19, well-established coagulopathy with the development of microthrombi requires constant activation of the fibrinolytic function. Furthermore, viral entry is modulated by receptors such as TMPRSS2, which is necessary in the oral cavity, leading to a derailed immune response that peaks in cytokine storm syndrome. This paper outlines the significance of the Plg/Pm system for infectious and inflammatory diseases that start in the oral cavity.

Awareness About Post Covid Complications In The Oral Cavity Among Dental Students

Introduction: Coronavirus is a large group of RNA viruses that causes chronic respiratory problems in humans. Severe COVID- 19 acute infection, along with associated therapeutic measures, could potentially contribute to various opportunistic fungal infection, xerostomia linked to decreased salivary flow, ulcerations and gingivitis as a result of impared immune system and susceptible oral mucosa. Aim(s): The aim of the study is to create awareness about post COVID complications in the oral cavity among dental students. Material(s) and Method(s): The sample size of the study was about 100 participants of undergraduate dental students. A set of questionnaires was created by the use of google form software, the data collection software score and represent the participants answer in a pie chart. Result(s): It is clear that 59 % of people were aware about the post COVID complications in the oral cavity.Among the males 38% of people were aware that gingivitis is a post COVID complication. Whereas, among females 19% of people were aware that gingivitis is a post COVID complication. Collectively among males and females, males were more aware about the post COVID complication in the oral cavity. Conclusion(s): The study had concluded that students were moderately aware about the post covid complications in the oral cavity. Copyright © 2022 Wolters Kluwer Medknow Publications. All rights reserved.

Assessment of Changes in the Microbiological Parameters of the Oral Fluid in Patients Who Underwent Sovid-19 with Complete Edentulism before and after Prosthetics

Organs such as the mouth, nose and eyes are the gateway for the coronavirus. If favorable conditions for the increase of infection are created in the oral mucosa with a decrease in immunity, the virus binds to the ACE-2 molecule (angiotensin-converting enzyme 2 membrane protein) and affects the epithelium. In the future, if the oral cavity is not protected, not only the mucous membrane of the oral cavity, but also the tissues around the teeth and the throat, as well as petechiae and stomach ulcers, can be observed [1, 2, 6, 7]. The process of development of ulcers in the oral cavity: painful inflammation of the papillae of the tongue (day), then the appearance of an erythematous spot (day), which turns into irregular and asymptomatic ulcers;therefore, many patients may be unaware of an oral ulcer due to the absence of pain (Chaux-Bodard et al., 2020). The burning sensation in the mouth (22.4%) could be due to several causes (eg, candida infection, dry mouth, mouth ulceration, or medications). A viral infection can weaken the immune system, causing secondary infections such as oral thrush. Candidiasis is the most common opportunistic infection in HIV (Nokta, 2008) and has also been reported in Covid-19 (dos Santos et al., 2020). The manifestation of oral lesions in patients with Covid-19 may be related to the direct or indirect effect of SARSCoV-2 on the oral mucosa, hypersensitivity to drugs taken during Covid-19 infection, the patient's susceptibility to the disease itself, or the duration of hospitalization. The SARS-CoV-2 virus infects human cells through angiotensin converting enzyme (ACE-2) receptors, since ACE-2 acts as the primary host cell receptor for this virus [4, 5, 8]. The oral cavity is an open biological system, in which there is a constant balance between pathogenic factors, such as viruses, bacteria, and the body's defenses. However, such an important balance is often attacked, both due to the multiplication and accelerated development of microorganisms and viruses, and due to the weakening of the very factors of general and especially local immune defense [3]. Immunoglobulins and lytic enzymes play an important role in the body's defense system;they are a component of immunity that-provides protection against pathogens (a wide range of bacteria, fungi, viruses), and also participate in the development of inflammation processes, maintenance and regulation of the adaptive immune system. One of the important components of the complex mechanism of innate immunity are neutrophils, when activated by pathogenic or opportunistic microorganisms, a wide range of anti-inflammatory cytokines is secreted. Copyright © 2022, Anka Publishers. All rights reserved.

Covid -19 aoosciated with Oral Mucosal Manifestation : A Review Article

The novel coronavirus disease 2019 is highly contagious disorder cause severe illness in form of fever, myalgia, fatigue, altered smell and taste sensation. The oral cavity manifestations has been discovered in Covid 19 patients. Studies shows that SARS-COV -2 affect respiratory track along with other organs those who have Angiotensin coverting enzyme 2 (ACE 2) receptors. ACE 2 Receptors are found in numerous amount in the oral cavity tissue which are more prone for SARS-COV-2 infections. There are various types of oral lesion associated with covid -19 patients such as ulcers in oral cavity, candidiasis, recurrent herpes simplex, geographical tongue, mucositis and petechiae. It affects oral cavity as well as salivary glands. This review article discusses the major neglected clinical entity such as oral cavity lesion in Covid -19 patients. Copyright © 2022 Wolters Kluwer Medknow Publications. All rights reserved.

Orofacial and facial pain during the new coronavirus infection (COVID-19) pandemic. Choosing a non-steroid anti-inflammator – focus on nimesulide

Doctors of various specialties face orofacial and facial pain in their practice: dentists, maxillofacial surgeons, neurologists, neurosurgeons, otorhinolaryngologists, therapists, general practitioners, traumatologists, ophthalmologists, psychotherapists, physiotherapists, aesthetic medicine doctors, anesthesiologists, plastic surgeons and other specialists. The novel coronavirus infec-tion (COVID-19) pandemic, spread by the SARS-CoV-2 virus, has become a challenge for all medical specialties and health care systems around the world. The respiratory system is the dominant target of SARS-CoV-2. The oral mucosa is also highly susceptible to SARS-CoV-2 infection, due to the wide distribution of angiotensin-converting enzyme type II receptors in the oral cavity. We searched the literature for the period from the beginning of the pandemic until August 22, 2022, on the study of the association of orofacial pain with a new coronavirus infection COVID-19 in the electronic search engines PubMed and Scopus. Analysis of the found sources indicates an increase in the frequency of various types of orofacial pain associated with 1) violation of dentoalveolar and related structures during the COVID-19 pandemic;2) temporomandibular disorders;3) with damage or disease of the cranial nerves;and 4) like manifestations of primary headache. This growth is due both directly to the infection caused by SARS-CoV-2, its complications, and to a complex of biopsychosocial factors directly related to the pandemic. In the second part of the article, as part of the review, the possibility of using nimesulide for the treatment of orofacial and facial pain during the COVID-19 pandemic is discussed. The choice of nimesulide is supported by 1) the likely coadjuvant effect of nimesulide in the treatment of COVID-19;2) positive experience with the use of nimesulide in the treatment of facial and orofacial pain in the period preceding the COVID-19 pandemic;3) the safety of using nimesulide in comorbid patients, who are the main risk group for the development and severe course of COVID-19. © 2022, Remedium Group Ltd. All rights reserved.

[Composition of oropharyngeal microbiota in patients with COVID-19 of different pneumonia severity].

AIM: To identify features of the taxonomic composition of the oropharyngeal microbiota of COVID-19 patients with different disease severity. MATERIALS AND METHODS: The study group included 156 patients hospitalized with confirmed diagnosis of COVID-19 in the clinical medical center of Yevdokimov Moscow State University of Medicine and Dentistry between April and June 2021. There were 77 patients with mild pneumonia according to CT (CT1) and 79 patients with moderate to severe pneumonia (CT2 and CT3). Oropharyngeal swabs were taken when the patient was admitted to the hospital. Total DNA was isolated from the samples, then V3V4 regions of the 16s rRNA gene were amplified, followed by sequencing using Illumina HiSeq 2500 platform. DADA2 algorithm was used to obtain amplicon sequence variants (ASV). RESULTS: When comparing the microbial composition of the oropharynx of the patients with different forms of pneumonia, we have identified ASVs associated with the development of both mild and severe pneumonia outside hospital treatment. Based on the results obtained, ASVs associated with a lower degree of lung damage belong predominantly to the class of Gram-negative Firmicutes (Negativicutes), to various classes of Proteobacteria, as well as to the order Fusobacteria. In turn, ASVs associated with a greater degree of lung damage belong predominantly to Gram-positive classes of Firmicutes Bacilli and Clostridia. While being hospitalized, patients with severe pneumonia demonstrated negative disease dynamics during treatment significantly more often. CONCLUSION: We have observed differences in the taxonomic composition of the oropharyngeal microbiota in patients with different forms of pneumonia developed outside hospital treatment against COVID-19. Such differences might be due to the presumed barrier function of the oropharyngeal microbiota, which reduces the risk of virus titer increase.

We don't know everything (we know) about dogs

This article highlights information on the beneficial and pathogenic microorganisms of the oral cavity of dogs, effects of grass consumption, and the breeding and care of dogs.